During which phase of mitosis do the microtubules attach to the kinetochores on the chromosomes?

During which phase of mitosis do the microtubules attach to the kinetochores on the chromosomes?

The Biology Project > Cell Biology > Intro. to Cell Cycle & Mitosis > Tutorial

Mitosis

What is (and is not) mitosis?

Mitosis is nuclear division plus cytokinesis, and produces two identical daughter cells during prophase, prometaphase, metaphase, anaphase, and telophase. Interphase is often included in discussions of mitosis, but interphase is technically not part of mitosis, but rather encompasses stages G1, S, and G2 of the cell cycle.

Interphase & mitosis
Interphase
During which phase of mitosis do the microtubules attach to the kinetochores on the chromosomes?


The cell is engaged in metabolic activity and performing its prepare for mitosis (the next four phases that lead up to and include nuclear division). Chromosomes are not clearly discerned in the nucleus, although a dark spot called the nucleolus may be visible. The cell may contain a pair of centrioles (or microtubule organizing centers in plants) both of which are organizational sites for microtubules.

Prophase
During which phase of mitosis do the microtubules attach to the kinetochores on the chromosomes?


Chromatin in the nucleus begins to condense and becomes visible in the light microscope as chromosomes. The nucleolus disappears. Centrioles begin moving to opposite ends of the cell and fibers extend from the centromeres. Some fibers cross the cell to form the mitotic spindle.

Prometaphase
During which phase of mitosis do the microtubules attach to the kinetochores on the chromosomes?


The nuclear membrane dissolves, marking the beginning of prometaphase. Proteins attach to the centromeres creating the kinetochores. Microtubules attach at the kinetochores and the chromosomes begin moving.

Metaphase
During which phase of mitosis do the microtubules attach to the kinetochores on the chromosomes?


Spindle fibers align the chromosomes along the middle of the cell nucleus. This line is referred to as the metaphase plate. This organization helps to ensure that in the next phase, when the chromosomes are separated, each new nucleus will receive one copy of each chromosome.

Anaphase
During which phase of mitosis do the microtubules attach to the kinetochores on the chromosomes?


The paired chromosomes separate at the kinetochores and move to opposite sides of the cell. Motion results from a combination of kinetochore movement along the spindle microtubules and through the physical interaction of polar microtubules.

Telophase
During which phase of mitosis do the microtubules attach to the kinetochores on the chromosomes?


Chromatids arrive at opposite poles of cell, and new membranes form around the daughter nuclei. The chromosomes disperse and are no longer visible under the light microscope. The spindle fibers disperse, and cytokinesis or the partitioning of the cell may also begin during this stage.

Cytokinesis
During which phase of mitosis do the microtubules attach to the kinetochores on the chromosomes?


In animal cells, cytokinesis results when a fiber ring composed of a protein called actin around the center of the cell contracts pinching the cell into two daughter cells, each with one nucleus. In plant cells, the rigid wall requires that a cell plate be synthesized between the two daughter cells.

Mitosis
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During which phase of mitosis do the microtubules attach to the kinetochores on the chromosomes?

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A cell is arrested during mitosis. At this stage, distinct chromatids are visible at opposite poles of the cell. Which stage of mitosis does this describe?

Possible Answers:

Anaphase

Metaphase

Prophase

Telophase

Explanation:

The phases of mitosis can be described as follows:

During prophase chromatin condenses to form discrete chromosomes.

During metaphase microtubules attach to the kinetochores and chromatids begin segregating.

During anaphase sister chromatids have been separated and reside at opposite poles of the cell.

During telophase both sets of chromatids are surround by new nuclear membranes and chromosomes decondense into chromatin.

Cytokinesis (the dividing of the cytoplasm into two cells) follows telophase.

If the cell were arrested during telophase, distinct chromatids would no longer be visible.

What is the correct order of the stages of mitosis?

Possible Answers:

Metaphase, telophase, anaphase, prophase

Prophase, metaphase, anaphase, telophase

Prophase, anaphase, telophase, metaphase

Anaphase, metaphase, prophase, telophase

Anaphase, metaphase, telophase, prophase

Correct answer:

Prophase, metaphase, anaphase, telophase

Explanation:

Mitosis is the process of cell division, and is separated into four stages. During prophase, the chromosomes condense and the nuclear envelope dissolves. During metaphase, the chromosomes align at the center of the cell. During anaphase, the sister chromatids are separated and pulled to opposite ends of the cell. During telophase, the nuclear envelope begins to re-form around the divided chromatids.

Which of the following events does not take place in prophase?

Possible Answers:

Chromosomes become visible in the cell

Centrioles move to the opposite ends of the cell

Sister chromatids are separated

The nucleolus disappears

Correct answer:

Sister chromatids are separated

Explanation:

Prophase is the first stage of mitosis, during which the cell begins to position itself in order to separate the chromatids and divide. During prophase, the nuclear envelope and nucleolus are dissolved and the chromosomes condense. The centrioles and spindle fibers begin to form at opposite poles of the cell.

Chromatid separation is not seen until anaphase.

What is the correct order of the steps of mitosis?

Possible Answers:

Anaphase, cytokinesis, metaphase, prophase, telophase

Anaphase, prophase, metaphase, telophase, interphase

Prophase, interphase, metaphase, anaphase, telophase

Prophase, metaphase, anaphase, telophase, cytokinesis

Correct answer:

Prophase, metaphase, anaphase, telophase, cytokinesis

Explanation:

There are five main steps of mitosis.

  • Prophase: the nuclear envelope breaks down, chromosomes condense, and spindle fibers connect to sister chromatids.
  • Metaphase: spindle fibers pull sister chromatids to the metaphase plate at the equator of the cell.
  • Anaphase: spindle fibers separate sister chromatids to opposite poles of the cell.
  • Telophase: chromatids reach the poles of the cell, the spindle fibers detach, and cleavage at the metaphase plate/equator begins to form.
  • Cytokinesis: the cell cytoplasm splits completely in half where the cleavage formed, giving two identical daughter cells with the same chromosomes as the parent.

Some scientists classify cytokinesis as its own event, separate from mitosis. Interphase is a portion of the cell cycle for growth, and is not a part of mitotic division.

In which phase of mitosis do chromosomes align at the center and spindle fibers become visible?

Possible Answers:

Telophase

Metaphase

Prophase

Anaphase

Interphase

Explanation:

Mitosis involves four distinct stages: prophase, metaphase, anaphase, and telophase.

Prophase is the first step of mitosis, during which chromosomes condense and the nuclear envelope dissolves. Metaphase follows prophase. During metaphase, the chromosomes align in the center of the cell at the equatorial plate and the spindle fibers attach to the centromeres of the chromosomes. Anaphase involves the retraction of the spindle fibers and the separation fo sister chromatids. The nuclear envelope begins to develop and the cell prepares for the final division during telophase. Cytokinesis is not generally included in the phases of mitosis, and refers to the final separation of the cell cytoplasm into two identical daughter cells.

Interphase refers to the portion of the cell cycle during which the cell is not dividing, and includes the G1, S, and G2 phases.

What is the correct sequence of the stages of the M phase of the cell cycle?

Possible Answers:

Metaphase, prophase, anaphase, telophase

Prophase, anaphase, metaphase, telophase

Telophase, anaphase, metaphase, prophase

Prophase, metaphase, anaphase, telophase

Correct answer:

Prophase, metaphase, anaphase, telophase

Explanation:

The mitosis (M) phase of the cell cycle has four sequential stages: prophase, metaphase, anaphase, and telophase. In the first stage, prophase, each chromosome becomes condensed and more visible, and there is the breakdown of the nuclear membrane and appearance of spindle fibers. In the next phase, metaphase, the chromosomes line up along the metaphasic plate. The spindle fibers are completely formed and are attached to each sister chromatid of the chromosomes. Next, during anaphase, the centromeres holding the sister chromatids together are divided and the sister chromatids are pulled apart by the spindle fibers to opposite poles of the diving cell. During the last phase of mitosis, telophase, the two sets of chromosomes reach the poles of the cell. This is followed by the reformation of the nuclear envelope, uncoiling of the DNA, and disappearance of the spindle fibers.

The division of centromeres occurs during which phase of mitosis?

Possible Answers:

Cytokinesis

Anaphase

Metaphase

Telophase

Prophase

Explanation:

Centromeres are the structures at the center of each chromosome that hold the sister chomatids together. The division of the centromeres occurs during anaphase. This allows for the separation of each sister chromatid into its respective daughter cell.

Mitosis has four sequential stages: prophase, metaphase, anaphase, and telophase. In prophase, each chromosome becomes condensed and more visible, and there is the breakdown of the nuclear membrane and appearance of spindle fibers. In the next phase, metaphase, the chromosomes line up along the metaphasic plate. The spindle fibers are completely formed and are attached to each sister chromatid of the chromosomes. Next, during anaphase, the centromeres holding the sister chromatids together are divided and the sister chromatids are pulled apart by the spindle fibers to opposite poles of the diving cell. During the last phase of mitosis, telophase, the two sets of chromosomes reach the opposite poles of the cell. This is followed by the reformation of the nuclear envelope, uncoiling of the DNA, and disappearance of the spindle fibers. After the M phase is the separation of the cytoplasm, known as cytokinesis, which results in two distinct daughter cells.

Which of the following events is characteristic of telophase in mitosis?

Possible Answers:

Breakdown of nuclear envelope

Formation of spindle fibers

Uncoiling of chromosomes

Separation of sister chromatids

Correct answer:

Uncoiling of chromosomes

Explanation:

Mitosis has four sequential stages: prophase, metaphase, anaphase, and telophase. In prophase, each chromosome becomes condensed and more visible, and there is the breakdown of the nuclear membrane and appearance of spindle fibers. In the next phase, metaphase, the chromosomes line up along the metaphase plate. The spindle fibers are completely formed and are attached to each sister chromatid of the chromosomes. Next, during anaphase, the centromeres holding the sister chromatids together are divided and the sister chromatids are pulled apart by the spindle fibers to opposite poles of the diving cell. During the last phase of mitosis, telophase, the two sets of chromosomes reach the opposite poles of the cell. This is followed by the reformation of the nuclear envelope, uncoiling of the DNA, and disappearance of the spindle fibers. After the M phase is the separation of the cytoplasm, known as cytokinesis, which results in two distinct daughter cells.

In which phase of mitosis does a cell utilize a specific control mechanism to verify its readiness for DNA synthesis?

Possible Answers:

Telophase

S

Prophase

G1

G2

Explanation:

The G1 phase is the first phase within interphase. It can more easily be remembered as the "first growth" phase. During this period the cell increases its supply of proteins while also increasing the number of organelles (such as ribosomes and mitochondria) in addition to growing in size. This stage is critical toward making sure that the cell has appropriate resources for DNA synthesis. After G1, S phase proceeds. 

In which phase has the cell left the cell cycle and has stopped dividing, in other words, which of the following is the resting phase?

Possible Answers:

G2 phase

S phase

G0

Telophase 

G2

Explanation:

G0 phase is a post-mitotic phase. It may be a permanent (as often is the case for neurons) or semi-permanent (for some liver, kidney, and stomach cells) phase. Epithelial cells do not enter G0, allowing these cells to continue to divide throughout an organisms life. 

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What phase do kinetochores attach to chromosomes?

At metaphase, the kinetochore, a proteinaceous multidomain structure, is assembled on the outer surface of the centromere, promoting attachment of the chromosome to spindle microtubules and movement during anaphase.

During which phase of mitosis are the kinetochore microtubules first attaching to the kinetochores of the sister chromatids?

During prometaphase, mitotic spindle microtubules from opposite poles attach to each sister chromatid at the kinetochore.

Where do microtubules attach to chromosomes during mitosis?

These microtubules are of three types, two of which make up the mitotic spindle. Kinetochore microtubules attach to the condensed chromosomes of mitotic cells at their centromeres, which are associated with specific proteins to form the kinetochore (see Figure 4.16).

During what stage of mitosis does the mitotic spindle attach to the kinetochores?

The stage of mitosis during which mitotic spindle fibres attach to kinetochores is called Prometaphase. During prometaphase, the nuclear membrane breaks down, allowing microtubles to interact with the condensed chromosomes. Each chromosome attaches to the microtubules via kinetochores.