What is one major difference between eukaryotes and prokaryotes with respect to translate multiple choice?

1. Most eukaryotic cells have multiple linear chromosomes, whereas prokaryotic cells have just one circular chromosome.

Since prokaryotic cells typically have only a single, circular chromosome, they can replicate faster than eukaryotic cells. In fact, a prokaryotic cell can undergo two rounds of DNA replication before the cell, itself, has divided. This means that DNA replication can occur during cell division in prokaryotes.

Since eukaryotic cells typically have multiple linear chromosomes, capped with telomeres, eukaryotic DNA replication and cell division (mitosis and meiosis) are a bit more complicated. In eukaryotic cells, DNA replication occurs before mitosis begins, and it can’t occur while the cell is dividing. In addition, the telomeres—repeating DNA sequences at the ends of each chromosome—limit the number of times a cell can divide before it dies or becomes senescent. Each time a typical or somatic eukaryotic cell divides, the telomeres get shorter.

2. Eukaryotic chromosomes are located within the nucleus, whereas prokaryotic chromosomes are located in the nucleoid.

The key difference between prokaryotic and eukaryotic cells is that eukaryotic cells have a membrane-bound nucleus (and membrane-bound organelles), whereas prokaryotic cells lack a nucleus. In eukaryotic cells, all the chromosomes are contained within the nucleus. In prokaryotic cells, the chromosome is located in a region of the cytoplasm called the nucleoid, which lacks a membrane.

One interesting implication of this difference in the location of eukaryotic and prokaryotic chromosomes is that transcription and translation—the processes of creating an RNA molecule and using that molecule to synthesize a protein—can occur simultaneously in prokaryotes. This is possible because prokaryotic cells lack a nuclear membrane, so transcription and translation occur in the same region. As the RNA is being transcribed, ribosomes can begin the translation process of stringing together amino acids. In contrast, in eukaryotic cells, transcription always occurs first, and it takes place within the nucleus. The RNA molecule needs to undergo editing before it leaves the nucleus. Then, translation is conducted by a ribosome in the cytoplasm.

3. In eukaryotic chromosomes, DNA is wound around histone proteins, and then, it is further compacted by supercoiling and folding. In prokaryotic chromosomes, DNA is supercoiled and compacted by nucleoid-associated proteins.

In general, eukaryotic cells contain a lot more genetic material than prokaryotic cells. For example, each human cell has around 2m, or 3 billion base pairs, of DNA that must be compacted to fit within the nucleus.

In eukaryotic cells, chromatin consists of all the DNA within the nucleus and its associated proteins, called histones. Eukaryotic chromosomes are composed of chromatin, and each consists of two complementary strands of DNA coiled tightly around histones. The structure of chromatin is scaffolded, with three distinct levels. First, the DNA is wrapped around clusters of histones, forming nucleosomes that are connected by linker DNA. Then, the linked nucleosomes undergo supercoiling. Like beads on a string, the connected nucleosomes loop around each other to form 30-nm solenoid fiber. The 30-nm chromatin fiber is compacted even further just before mitosis occurs, and its coils fold and compress into a 250nm-wide fiber.

Prokaryotic cells also have a lot of DNA, but the molecules don’t need to be packaged up quite as tightly as they do in eukaryotic cells. E. coli has around 1.6mm, or 4 million base pairs, of DNA—compare this to the 2m of DNA inside each human cell.

Although most prokaryotic cells don’t use histones to coil up their DNA, they have various proteins and enzymes that introduce folds to produce a complex, compacted structure.

Here is a basic summary of the key differences between eukaryotic and prokaryotic chromosomes.

The distinction between prokaryotes and eukaryotes is considered to be the most important distinction among groups of organisms. Eukaryotic cells contain membrane-bound organelles, such as the nucleus, while prokaryotic cells do not. Differences in cellular structure of prokaryotes and eukaryotes include the presence of mitochondria and chloroplasts, the cell wall, and the structure of chromosomal DNA.

Prokaryotes were the only form of life on Earth for millions of years until more complicated eukaryotic cells came into being through the process of evolution.

Comparison chart

Definition of eukaryotes and prokaryotes

Prokaryotes (pro-KAR-ee-ot-es) (from Old Greek pro- before + karyon nut or kernel, referring to the cell nucleus, + suffix -otos, pl. -otes; also spelled "procaryotes") are organisms without a cell nucleus (= karyon), or any other membrane-bound organelles. Most are unicellular, but some prokaryotes are multicellular.

Eukaryotes (IPA: [juːˈkæɹɪɒt]) are organisms whose cells are organized into complex structures by internal membranes and a cytoskeleton. The most characteristic membrane bound structure is the nucleus. This feature gives them their name, (also spelled "eucaryote,") which comes from the Greek ευ, meaning good/true, and κάρυον, meaning nut, referring to the nucleus. Animals, plants, fungi, and protists are eukaryotes.

Differences Between Eukaryotic and Prokaryotic Cells

The difference between the structure of prokaryotes and eukaryotes is so great that it is considered to be the most important distinction among groups of organisms.

• The most fundamental difference is that eukaryotes do have "true" nuclei containing their DNA, whereas the genetic material in prokaryotes is not membrane-bound.

• In eukaryotes, the mitochondria and chloroplasts perform various metabolic processes and are believed to have been derived from endosymbiotic bacteria. In prokaryotes similar processes occur across the cell membrane; endosymbionts are extremely rare.

• The cell walls of prokaryotes are generally formed of a different molecule (peptidoglycan) to those of eukaryotes (many eukaryotes do not have a cell wall at all).

• Prokaryotes are usually much smaller than eukaryotic cells.

• Prokaryotes also differ from eukaryotes in that they contain only a single loop of stable chromosomal DNA stored in an area named the nucleoid, while eukaryote DNA is found on tightly bound and organised chromosomes. Although some eukaryotes have satellite DNA structures called plasmids, these are generally regarded as a prokaryote feature and many important genes in prokaryotes are stored on plasmids.

• Prokaryotes have a larger surface area to volume ratio giving them a higher metabolic rate, a higher growth rate and consequently a shorter generation time compared to Eukaryotes.

• Genes
  • Prokaryotes also differ from eukaryotes in the structure, packing, density, and arrangement of their genes on the chromosome. Prokaryotes have incredibly compact genomes compared to eukaryotes, mostly because prokaryote genes lack introns and large non-coding regions between each gene.
  • Whereas nearly 95% of the human genome does not code for proteins or RNA or includes a gene promoter, nearly all of the prokaryote genome codes or controls something.
  • Prokaryote genes are also expressed in groups, known as operons, instead of individually, as in eukaryotes.
  • In a prokaryote cell, all genes in an operon(three in the case of the famous lac operon) are transcribed on the same piece of RNA and then made into separate proteins, whereas if these genes were native to eukaryotes, they each would have their own promoter and be transcribed on their own strand of mRNA. This lesser degree of control over gene expression contributes to the simplicity of the prokaryotes as compared to the eukaryotes.

References

• Wikipedia:Eukaryote
• Wikipedia:Prokaryote

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