Author affiliations * Corresponding authors a Faculty of Pharmacy, The
University of Sydney, Sydney, NSW 2006, Australia b CSIRO Manufacturing, Research Way, Clayton, VIC 3168, Australia c Microbiology Department, Freeman Hospital, Newcastle upon Tyne, UK d Sunderland
Pharmacy School, University of Sunderland, Sunderland, UK e bioMérieux Inc., Durham, NC, USA In order to retard the rate of development of antibacterial resistance, the causative agent must be identified as rapidly as possible, so that directed patient treatment and/or contact precautions can be initiated. This review highlights the challenges associated with
the detection and identification of pathogenic bacteria, by providing an introduction to the techniques currently used, as well as newer techniques that are in development. Focusing on the chemical basis for these techniques, the review also provides a comparison of their advantages and disadvantages.
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DOI
//doi.org/10.1039/C6CS00693KArticle type
Tutorial ReviewSubmitted
23 Sep 2016First published
23 Jun 2017Download Citation
Chem. Soc. Rev., 2017,46, 4818-4832
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Methods for the detection and identification of pathogenic bacteria: past, present, and future
L. Váradi, J. L. Luo, D. E. Hibbs, J. D. Perry, R. J. Anderson, S. Orenga and P. W. Groundwater, Chem. Soc. Rev., 2017, 46, 4818 DOI: 10.1039/C6CS00693K
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journal article
Current molecular techniques for the detection of microbial pathogensScience Progress (1933-)
Vol. 90, No. 1 (2007)
, pp. 29-50 (22 pages)
Published By: Sage Publications, Ltd.
//www.jstor.org/stable/43423199
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Abstract
Traditionally the detection of microbial pathogens in clinical, environmental or food samples has commonly neededtheprelevation of cells by culture before the application of the detection strategy. This is done to increase cell number thereby overcoming problems associated with the sensitivity of classical detection strategies. However, culture—based methods have the disadvantages of taking longer, usually are more complex and require skilled personnel as well as not being able to detect viable but non cultivable microbial species. A number of molecular methods have been developed in the last 10 to 15 years to overcome these issues and to facilitate the rapid, accurate, sensitive and cost effective identification and enumeration of microorganisms which are designed to replace and/or support classical approaches to microbial detection. Amongst these new methods, ones based on the polymerase chain reaction and nucleic acid hybridization have been shown to be particularly suitable for this purpose. This review generally summarizes some of the current and emerging nucleic acid based molecular approaches for the detection, discrimination and quantification of microbes in environmental, food and clinical samples and includes reference to the recently developing areas of microfluidics and nanotechnology "Lab-on-achip".
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Science Progress commissions world authorities to contribute articles on the most interesting, important and meaningful topics - ranging from cosmology to the environment, and publishes occasional issues on specific topics.
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SAGE Publications is an academic and professional publisher. We publish books, journals and software under the SAGE, Corwin Press, Paul Chapman Publishing, Pine Forge Press, SAGE Reference, SAGE Science and Scolari (US and Europe websites) imprints.
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Science Progress (1933-) © 2007 Sage Publications, Ltd.
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